hb 8730 mouse hybridoma cell line  (ATCC)

Journal: Kidney360

Article Title: NF Erythroid 2-Related Factor 2 Deficiency Enhances the Immunosuppressive Function of Monocytic Myeloid-Derived Suppressor Cells in a Peroxisome Proliferator-Activated Receptor- γ -Dependent Manner

doi: 10.34067/KID.0000000947

Figure Lengend Snippet: Nrf2 deficiency promotes the immunosuppressive function of M-MDSCs in vivo . B16-F10 melanoma cells were s.c. injected into WT and Nrf2 −/− mice. Spleens and tumors were harvested at 14 days postinjection. (A) The tumor size was calculated. (B) Tumor tissues were sectioned for histologic examination. Scale bar: 200 μ m. (C) CD4 + and CD8 + T-cell infiltration in the tumors was examined by IHC staining. Scale bar: 50 μ m. (D and E) The percentage of CD11b + cells (D), Ly6G − Ly6C hi M-MDSCs, and Ly6G + Ly6C lo G-MDSCs gated on CD11b + cells (E) from spleens or tumors were analyzed by flow cytometry. (F and G) The percentage of G-MDSCs and M-MDSCs from the total MDSCs (F) and the G-MDSC/M-MDSC ratio (G) in the spleens and tumors were determined ( n =6). (H) G-MDSCs and M-MDSCs sorted from tumors collected from WT or Nrf2 −/− mice were cultured with CFSE-labeled CD4 + T-cells stimulated with ConA. T-cell proliferation was measured after 3 days ( n =3). (A, C, D and H) Representative images are shown on the left and the summarized results are presented as the mean± SEM on the right ( n =6). * P < 0.05. H&E, hematoxylin and eosin; HPF, high-power field; IHC, immunohistochemical.

Article Snippet: The mouse melanoma cell line, B16-F10, was purchased from the American Type Culture Collection and was routinely cultured in high glucose DMEM (Gibco, NY) supplemented with 10% FBS (Life Technologies, Carlsbad, CA) at 37°C and 5% CO 2 .

Techniques: In Vivo, Injection, Immunohistochemistry, Flow Cytometry, Cell Culture, Labeling, Immunohistochemical staining